Yes, as long as it is diluted and your dog is prevented from ingesting it. Normally, one would clip the area around the hot spots very short so to allow the area to dry and would apply a solution mix of Betadine solution and warm water, made to look like weak tea. The area would then be treated twice daily. This is an antibacterial. Consider though that this solution can stain horribly so you probably want to do this outside. An Elizabethan collar would be important to prevent licking the area. Also, because hot spots are super painful, you may want your vet to shave the hot spots. When in pain, dogs can bite. Of course, hot spots with an infection require antibiotics prescribed by the vet. Please see your vet for proper diagnosis and treatment as there are other skin conditions that mimic hot spots.